Marine litter is an increasing threat to the marine environment. The importance of mapping it with the aim of detecting potential hotspots and preventing their spread is becoming increasingly important. In this paper, the applicability of the Chasing M2 remotely operated underwater vehicle (ROV) and Wasp S3 multi-beam echo sounder (MBES) in mapping - detecting and quantifying marine litter in the wider area of the St. Anthony's Channel (Šibenik), near the Martinska Research Station (Ruđer Bošković Institute), is investigated. It's been shown that the ROV Chasing M2 can be used effectively for the initial detection of marine debris in shallow waters. However, if the ROV's underwater navigation system and auxiliary measurement scales aren't used, the ROV has limited capabilities in quantifying marine debris on the seabed. The named was determined by comparing the morphometric parameters of the tire derived from the 3D model with a manual 3D scanner. According to the results, the WASSP S3 multi-beam echo sounder and backscatter sensor aren't suitable for identifying micro and meso marine debris on the seabed at depths up to 10 meters. It was found that the used MBES Wasp S3 can detect marine debris with an area of 100 * 100 centimeters and a height of about 40 centimeters at depths of up to 10 meters. In addition, MBES interval measurements have shown that WASSP S3 has a measurement accuracy in the centimeter range (< 10cm) at different cruising speeds. The results obtained have helped to establish the guidelines for the use of MBES and ROV in the detection of marine debris on the seabed.