The researches have been wondering for a long time what attracts such large audience to watch and enjoy the horror movies if they cause negative emotions such as fear and anxiety. Although this area is not completely researched, scientists have come to the conclusion that those who enjoy horror movies are higher on the dimension of sensation seeking, and that they watch them to experience strong positive emotions in the end. Studies have also shown that the experience of fear is more intense using virtual reality compared to the computer screen so this new technology is used even in this study. Due to the lack of paperwork in this field, the aim of this study was to investigate some differences in habituation to horror content between people who love horror movies and people who do not like them. The study included 56 participants (43 who love horror moviesand 13 who don't like horror movies). The participants went through the same horror simulation three times using virtual reality device while being attached to electrocardiograph. The R-R intervals, heart rate variability operationalised as standard deviation of R-R intervals, and Thayer's activation-deactivation adjective check list were used as a measure of habituation. Results show larger R-R intervals in both groups in the third measurement compared to the first, which is an evidence for physiological habituation. Standard deviation of R-R intervals showed no effects. Within participants that don't like horror movies, activation assessment is significantly greater after the first simulation in comparison with the third simulation, but also compared to subjects who love horror movies. It was also found that within participants who love horror movies there is no difference in activation assessment in the first and the third measurement. Correlation was found between standard deviation of R-R intervals and activation assessment during the first measurement within participants that don't like horror movies.