The goal of this research was to examine the relationship between 2D:4D ratio and jealousy in men and women. 2D:4D ratio represents the ratio of index finger length and ring finger length. It is believed that this phenotypical characteristic represents prenatal androgen exposure and correlates with sexually dimorphic behaviours. One of sexually dimorphic reactions is jealousy which can be defined as arousal, caused by a perceived threat, that serves for protection, maintenance and extension of one's relationship with a partner, as well as countering the threat. Research has shown that men experience more sexual jealousy and women experience more emotional jealousy, which makes this construct a potential correlate of 2D:4D. Knowing the relationship between 2D:4D and jealousy can be helpful in understanding biological mechanisms that are at the core of between-sex and within-sex differences in jealousy. Students from University of Zadar (N=100), aged 18 to 28, took part in this study. General information was collected with a questionnaire made specifically for this study. The digit ratio was measured with a digital caliper, and jealousy was tested with scenarios of sexual and emotional jealousy. Results show that the 2D:4D ratio of men's left hand is significantly lower than the 2D:4D ratio of left hands in women. Furthermore, there is no sex difference in the 2D:4D ratio of participants' right hand. No differences were found between sexual jealousy between sexes. However, the results show that women are more emotionally jealous than man. There was no significant difference between sexual and emotional jealousy within sexes. Lastly, the relationships between 2D:4D digit ratio and jealousy in men and women were not significant in this study.