The aim of the study: To examine the frequency of occurrence of multi-resistant microorganisms (MDR) in the surveillance cultures of patients hospitalized in the ICU of the Clinic for Anesthesiology, Reanimation and Intensive Care of the Clinical Hospital Centre Split in 2023, taking into account different characteristics of the subjects such as age, gender, comorbidities and applied therapeutic procedures. Materials and methods: Data were collected on the state of health of 53 patients who, upon admission, had positive results of surveillance cultures for multi-resistant microorganisms. The analysis was done in the statistical software STATISTICA 12 manufactured by Tibco, California. Results: A large proportion of respondents, 92.31% of them, had chronic comorbidities, with statistically significant differences. Of the total number of patients, 73.58% survived, while 26.42% died. A significant epidemiological data for the development of antimicrobial resistance is that before being admitted to the ICU, 69.81% of patients had already received antibiotics. The most common isolates include Escherichia coli ESBL from the rectum, Staphylococcus aureus MRSA from the nose and Pseudomonas aeruginosa from the tracheal aspirate. Also, Staphylococcus epidermidis MRSE was most often isolated from blood cultures. Discussion: Multiple resistance of microorganisms to antimicrobial drugs is becoming an increasingly common phenomenon, which leads to complications in the treatment of infections and increased mortality among hospitalized patients in the ICU. Conclusion: The results of post-decolonization rectal, nasal and axillary swabs showed a high percentage of successful decolonization, although a statistically significant association between pre-decolonization and post-decolonization swab results was not established