Introduction: The use of personal protective equipment is extremly important for the preservation of health and safety of employees in healthcare institutions. Personal protective equipment not only protects healthcare workers from various infections, it also has an important role in performing quality patient care by reducing the further spread of infectious diseases. Objective: This paper will present the role of the nurse in the proper use of personal protective equipment. Discussion: This paper will show the importance of timely application of personal protective equipment by nurses, and the necessity of education about it. Also, hand hygiene will be highlighted as the most important measure to prevent infections related to health care. Conclusion: Nurses have a key role in the use of personal protective equipment, as well as in educating others about it. Their role is necessary to successfully control and prevent infections. One of the most important thing in achieving this goal is the education of patients and their families, and successful communication with the rest of the healthcare team.