In the Croatian population, cancer is the second most important cause of death, right after cardiovascular diseases. Approximately a quarter of the deaths are caused by some form of cancer. In the countries of the European Union, including Croatia, breast cancer is the most frequently diagnosed malignant disease in women, and it can also occur in men. It is assumed that in the coming period, there will be an additional increase in the number of people suffering from malignant diseases, including breast cancer, due to the rise in life expectancy and a larger number of inhabitants at the age when the appearance of malignant diseases occurs more often. Compared to some other malignancies disease, and with the help of effective preventive and screening programs, it is possible to detect breast cancer at an early stage when it is not yet palpable and when the possibilities of healing are high. In the Republic of Croatia, in addition to the National Program for Early Detection of Colon Cancer and Cervical Cancer, the National Program for Early Detection of Breast Cancer has been implemented since 2006. The breast cancer screening program is scientifically based, profitable in the long term, and built on examples of good practice. Considering the expected contribution of the National Preventive Program to reducing the mortality rate as well as the contribution to the quality of life of patients through early and timely diagnosis, this paper aims to show the prevalence of breast cancer in the period from 2016 to 2021 and indirectly the effectiveness of the implementation of the national preventive program.