During last two years, since the COVID-19 pandemic arose, we can say that the rapid discovery of the coronavirus vaccine has divided the world. We had the opportunity to hear all kinds of stories around us. Also, we participated in many discussions that resulted in the presentation of both positive and negative attitudes about coronavirus vaccination and in general. It is for this reason that this study was conducted in which differences in the attitudes of Nursing students about vaccination against COVID-19 disease were examined. The aim of this paper was to examine the differences in attitudes of Nursing students on vaccination against COVID-19 disease with respect to the year of study. The survey was conducted in the period from April 4 to May 4, 2022. The survey was conducted through the online platform Google Forms and was completely anonymous. It consists of 40 questions. It was modified according to the Wang et al. survey questionnaire (22). The questions were grouped in such a way that one group of questions provided general information about the respondents, another group about student vaccination, a third that described students 'opinions on possible COVID-19 infection, a fourth that spoke about students' attitudes towards COVID-19 vaccination. The penultimate group of questions is focused on students' health literacy, while the last 2 questions indicate crucial decisions of students when deciding whether to be vaccinated. 271 respondents participated in the research. Of these, 231 (85.2%) female students and 40 (14.8%) undergraduate and graduate students of Nursing in the Republic of Croatia. The obtained research results are very valuable because they give us an insight into the attitudes of Nursing students about vaccination against the COVID-19 disease. Defining attitudes when it comes to the efficacy and safety of vaccines and possible side effects should be one of the activities aimed at these students so that they can make decisions based on objectively acquired knowledge based on the knowledge they possess or have passed on to them.