Introduction: Continuous and irregular change of shifts during shift work is closely related to depression and anxiety, which causes a higher number of sick days and leaving the nursing profession. Objective: To examine the connection between shift work and the chronotype of employed nurses/technicians in the General Hospital of Šibenik-Knin County with the intention to leave the nursing profession. Materials and methods: 111 employed nurses and technicians participated in the research. The research instrument was a survey questionnaire that contained sociodemographic and general questions, the Turnover intention scale (TIS-6) of six questions, and the Composite Scale of Morningness (CSM) of 13 questions. Results: The most common form of work was working in a 12-hour shift (59.46%), and the least common was working in the morning and afternoon shifts (4.5%). The participants chose salary and interpersonal relationships as the strongest motivation factor. The highest values on the TIS-6 scale were recorded in participants who work morning shifts (TIS = 18.57), and the lowest in those who alternate between morning and afternoon shifts (TIS = 14.60). According to the chronotype, the majority of the participants were of the intermediate type (74.78%), and the least of the evening type (2.7%). Mean values on the TIS-6 scale are similar for intermediate (TIS = 17.53) and morning (TIS = 16.40) participants, and highest (TIS = 25) for evening type participants. Participants who belonged to the evening type significantly differed in their intention to leave the profession compared to those who belonged to the intermediate (p = 0.024) and morning type (p = 0.025). No significant differences were found between intermediate and morning type participants (p = 0.402). Conclusion: The results confirm the connection between the form of work and the employee's chronotype with the intention to leave the profession, i.e. with staying at work.