The topic of this thesis is the development of Glagolitic alphabet and its popularization in preschool institutions. The historical development of the Glagolitic alphabet, which has its significance for the development of the Croatian alphabet, is explained in detail. Also, as part of the project, empirical research in kindergarten was proven with the aim of teaching children about the Glagolitic alphabet and its historical significance. Glagolitic is the first Croatian alphabet and one of the features of Croatian cultural identity. There is still controversy about the origin of the Glagolitic alphabet, so it is very useful to reconsider generally accepted theories and beliefs. About the origin of the Glagolitic alphabet historians know about forty different theories. In order to popularize the Glagolitic alphabet in kindergarten, I will present an example of proven qualitative research. The research is based on proven activities, worksheets and conducting various exercises, all with the purpose of collecting data on the way kindergartens participate in the Glagolitic alphabet of their children.