INTRODUCTION. Recent scientific studies have once again highlighted the importance of the hospital environment in the prevention and control of infections. However, many healthcare facilities still do not consider environmental hygiene crucial for patient safety. For example, inadvertent exposure to opportunistic pathogens from the environment can result in infections with significant morbidity and/or mortality. Several high-quality studies have linked the implementation of environmental hygiene interventions in healthcare settings to a reduction in either patient colonization with epidemiologically relevant pathogens or healthcare-associated infections. AIM AND OBJECTIVE. To demonstrate the role of nurses in implementing nursing interventions in the field of environmental hygiene, which affect patient safety. DISCUSSION. Nurses play a key role in enforcing prescribed hygiene standards, as well as in supervising the work and educating staff responsible for the process of cleaning and disinfecting the hospital environment. Implementing clearly defined cleaning protocols and regularly monitoring their effectiveness can significantly reduce the risk of healthcare-associated infections. Staff responsible for organizing the maintenance of environmental hygiene in healthcare settings must be continuously educated by following new scientific insights and improving cleaning practices, as well as introducing new methods and technologies for better infection control. CONCLUSION. Maintaining cleanliness and hygiene in healthcare facilities, in addition to hand hygiene, is crucial for the prevention of hospital infections. Therefore, it is necessary to conduct continuous education and practical training of staff to ensure patient safety from the aspect of a microbiologically safe hospital environment.