Introduction: Organ transplantation belongs to the group of modern methods of surgical treatment of patients through the replacement of organs or tissues that have been diagnosed with failure or complete cessation of functions. It is defined as the surgical procedure of transplanting an organ or tissue from one organism to another. It includes procedures such as donation and procurement, testing, preservation, storage, allocation and distribution of organs and tissues. Aim: To examine the knowledge and attitudes of nurses employed at Zadar General Hospital about organ donation. Materials and methods: Nurses and medical technicians employed at Zadar General Hospital participated in the research. The instrument used in this research, in addition to the questionnaire on general demographic data, are items taken from a standardized questionnaire designed by Chung et al. (2008). The research was conducted cross-sectionally, online via a Google forms form in the period from July to September 2023. Results: 119 female respondents and 15 male respondents participated in the research. A higher number of positive attitudes compared to negative attitudes and a higher number of incorrect answers compared to correct answers about organ donation among nurses and technicians of Zadar General Hospital were determined. No statistically significant difference was found in knowledge about organ donation with regard to gender, age, marital status and level of education, while a statistically significant difference was found in knowledge about organ donation in certain areas with regard to work experience and attending education about organ donation. No statistically significant difference was found in attitudes about organ donation with regard to gender and marital status, while a statistically significant difference was found in certain attitudes of nurses and technicians about organ donation with regard to age, level of education, work experience and attendance at organ donation education. A statistically significant positive correlation was found between attitudes and knowledge about organ donation. Conclusion: On the basis of the recorded results, a generally positive attitude and a lower level of knowledge of nurses and technicians employed at the Zadar General Hospital regarding organ donation was determined. Education of nurses and technicians is needed in order to improve the quality of work with patients and to develop positive attitudes about organ donation in the general public.