Introduction: Current scientific evidence indicates that a contaminated environment plays an important role in the spread of microorganisms, and if not thoroughly cleaned and/or disinfected on a regular basis, then it can act as a reservoir of potential pathogens (1). The aim of the research: was to determine the share of cleaned surfaces in the patient rooms of the Zadar General Hospital and to compare the obtained results with the previous research. Respondents and methods: Respondents are cleaners (N-70) who are assigned to cleaning processes in Zadar General Hospital, regardless of age, gender and length of work experience. As a measuring instrument, the prescribed form of the company Ecolab d.o.o. was used for recording the process of marking surfaces and evaluating cleaned/uncleaned surfaces. Fluorescent gel and UV lamp were used for the marking process to evaluate the correct method and cleaning process. Results: Patient rooms in the departments of pediatrics (25%), neurology (22%) and gynecology (32%) had the lowest percentage in the assessment of cleanliness. According to the results, the Department of Neurosurgery has the lowest percentage of cleanliness (50%), followed by the Department of Vascular Surgery. The trauma department has the highest percentage of cleanliness (90%). In the Department of Internal Diseases, the Department of Gastroenterology was rated as the cleanest Department (80%), and the Department of Pulmonology had the lowest degree of cleanliness with only 48% of clean surfaces. The total thoroughness of cleaning in the observed period was 22.9%, which is 40% less compared to the research that was conducted now. Conclusion: The share of cleaned surfaces in the patient rooms of Zadar General Hospital still does not show large numbers, but the results are still better compared to the previous research.