A quality relationship between co-workers is one of the key factors of every organization, including preschool education institutions. The main goal of the research was to gain insight into the assessment of precshool teacher ant the relationship between them and professional associates; pedagogue, psychologist, health manager and expert in educational and rehabilitation profile (educational rehabilitator, speech therapist, social pedagogue). At the beginning of the work, relationships are defined, what contributes to their quality, how teamwork works in kindergarten and what are the possible barriers. Furthermore, the roles of all professional associates are presented and their cooperation with educators is described. At the end of the paper, the results of the research conducted in four counties, Zadar, Karlovačka, Zagrebačka and the City of Zagreb, are presented, which show that preschool teacher often encounter several problems, ie. barriers that hinder them in their desire for a quality relationship with professional colleagues. Most often, these are the desire for a greater presence of professional associates in educational groups, an equal relationship, a desire for honest and open communication, greater openness and respect for suggestions, and greater support in working with parents.