The paper deals with the influence of ESI funds on the development of entrepreneurship on the example of Požega-Slavonia County. As a full member, the Republic of Croatia has the possibility to use EU funds. That enabled Croatia to make great progress, especially due the cofinancing of projects related to the encouragement of entrepreneurship and the development of entrepreneurial infrastructure. The European Union contributes to the encouragement and development of entrepreneurship through various measures and policies. Education and workshops on this topic are indispensable, because in this way, through exercises and experiences, participants get the best idea about entrepreneurship itself, entrepreneurial processes and decisions. ESI funds are collective names for the following five funds: European Regional Development Fund, European Social Fund, Cohesion Fund, European Maritime and Fisheries Fund and European Agricultural Fund for Rural Development. With the example of the Požega-Slavonska County, the European Regional Development Fund (ERDF), which financed projects such as the E-incubator in Pleternica, was particularly highlighted. Although the Požega-Slavonia County has a trend of population emigration, the programs and reports planned for the coming years are aimed at encouraging entrepreneurship and craftsmanship, motivating young people to turn to entrepreneurial ventures to create a positive entrepreneurial climate. Through quality and innovative programs and projects that are implemented, Požega- Slavonia County strengthens entrepreneurship and its economy as a whole.