INTRODUCTION: Hand hygiene is a mandatory, standardized procedure in nursing practice and basic measures for preventing healthcare associated infections. The World Health Organization has developed for all healthcare professionals the Hand Hygiene Guidelines, which include the term "My Five Moments for Hand Hygiene" and a hand hygiene protocol. In order to raise awareness of the health professionals about the importance of proper hand hygiene in health institutions in our country, national hand hygiene guidelines have been developed. Continuous education, secured technical conditions and acceptable hand hygiene tools contribute to better hand hygiene. OBJECTIVE: Show the role of nurse in the implementation of hand hygiene guidelines at the Department of Emergency Medicine of the County of Zadar. DISCUSSION: The Department of Emergency Medical Assistance of the County of Zadar is a health institution that provides ambulance / rehabilitation facilities, on-site and during transportation, providing health care for patients with urgent medical help and for people who are not endangered. Hygiene is an indispensable, basic procedure for nurses and emergency nursing technicians in the provision of health education. Medical nurses / technicians applying hand hygiene guidelines, guided by the principle of nurses ethics and collaborating with members of the emergency team, contribute to the quality of healthcare and care of outpatient emergency patients, patient and personal safety, hand hygiene, prevention of healthcare associated infections , reputation, and cost reduction of institutions. CONCLUSION: The nurse's role in the implementation of hand hygiene guidelines contributes to the prevention of infections that are transmitted by impure hands. Medical nurses / emergency technicians are familiar with the hand hygiene indices, while performing health care procedures apply routine and sanitary hand wash procedures, and hygienic laundry in their hands. Due to the generally known discrepancies between knowledge and behavior, it is essential to recall the importance of implementing the prescribed guidelines.